A VALIDATED LC METHOD FOR DETERMINATION OF CARBAMAZEPINE IN SALIVA

Carbamazepine (CBZ) is a drug used as anticonvulsant, especially in the treatment of epilepsy. For adequate control disease, must achieve blood concentrations, therefore, it very important to levels anticonvulsant drugs biological matrices with suitable analytical methods. A simple and fast liquid chromatographic method UV detection was developed validated for determination CBZ saliva. Chromatographic separation achieved RP-18 column, using acetonitrile water triethylamine at pH 7,3 mobile phase isocratic elution mode, flow rate 1 mL/min. The done 230 nm. bromazepam 1,5 μg/mL IS. run time 5 min. described linear over range 0,5 - 5,5 μg/mL. intraassay interassay precision, expressed RSD, were 0,67 2,93 % 0,17 9,33 %, respectively, extraction recoveries between 100,67 – 100,69 accuracy values ranged from −3,33 0,69 2,67 0,72 intraday interday analysis, assay demonstrated selectivity specificity. LLOQ below therapeutic level, demonstrating an sensitivity method. results showed that proposed found be quantitative